期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 48, 期 5, 页码 1196-1201出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2010.02.010
关键词
Mud crab; Simulated gastric fluid; Simulated intestinal fluid; Digestibility; Tropomyosin; Western blot
资金
- National Natural Scientific Foundation of China [20872049, 30871947]
- Ministry of Science and Technology of China [2008BAD94B01, 2008BAD94B09]
- Natural Scientific Foundation of Fujian Province [2008J0067]
- Foundation for Innovative Research Team of Jimei University [2006A002]
Stability in simulated gastric fluid is regarded as an important parameter for the estimation of food allergenicity. In this study, the digestive stability of allergenic protein tropomyosin (TM) and other food proteins from mud crab in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) digestion assay system was investigated and compared by SOS-PAGE and Western blot. In SGF system, proteins such as actin and the original band of myosin heavy chain (MHC) were rapidly degraded within a short period of time, while TM was relatively resistant to pepsin digestion. In SIF system, MHC was easily decomposed, while TM and actin were similarly resistant to digestion. Further study by IgE-immunoblotting and inhibition ELISA using sera from crab-allergic patients indicated that allergenicity of TM was partially decreased, suggesting proteinase digestion is effective in reducing the allergenicity of crab TM. (C) 2010 Elsevier Ltd. All rights reserved.
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