期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 48, 期 6, 页码 1734-1740出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2010.04.001
关键词
Aflatoxin B-1; Aspergillus flavus; Citrus maxima; Citrus sinensis; DL-Limonene; Essential oil
资金
- Council of Scientific and Industrial Research (CSIR) New Delhi, India
The study deals with antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima and Citrus sinensis essential oils (EOs) and their phytochemical composition. The EOs were obtained by hydrodistillation and their chemical profile was determined through GC and GC-MS analysis. Both the EOs and their 1:1 combination showed broad fungitoxic spectrum against different food contaminating moulds. The EOs and their combination completely inhibited aflatoxin B-1 (AFB(1)) production at 500 ppm, whereas, DL-limonene, the major component of EOs showed better antiaflatoxigenic efficacy even at 250 ppm. Both the oils exhibited antioxidant activity as DPPH free radical scavenger in dose dependent manner. The IC50 for radical scavenging efficacy of C maxima and C. sinensis oils were to be 8.84 and 9.45 mu l ml(-1), respectively. The EOs were found non-mammalian toxic showing high LD50 for mice (oral, acute). The oils may be recommended as safe plant based antimicrobials as well as antioxidants for enhancement of shelf life of food commodities by checking their fungal infestation, aflatoxin production as well as lipid peroxidation. (C) 2010 Elsevier Ltd. All rights reserved.
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