期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 47, 期 1, 页码 260-266出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2008.11.015
关键词
Antioxidants; Antigenotoxicity; Bacterial reverse mutation assay; Comet assay; Human cells in vitro; Monoterpenes
We studied the protective effect of Monoterpenes myrcene, eucalyptol and linalool against t-butyl hydroperoxide (t-BOOH) induced genotoxicity in reverse mutation assay with Escherichia coli WP2 IC185 strain and its oxyR mutant IC202, and with the comet assay in human hepatoma HepG2 and human B lymphoid NC-NC cells. The monoterpenes were tested in concentration ranges 0.05-1.5 mg/plate and 0.01 - 1.0 mu g/ml in bacteria and mammalian cells, respectively. Suppression of t-1300H induced mutagenesis was detected only in IC202 strain, and correlated with the observed inhibition of lipid peroxidation by the three monoterpenes. Linalool and myrcene strongly suppressed t-1300H induced mutagenesis. Eucalyptol, in addition to moderate suppression of t-1300H induced mutagenesis, suppressed also spontaneous mutagenesis. In NC-NC cells linalool and myrcene reduced t-1300H induced DNA damage by about 50% at 0.01 mu g/ml, while eucalyptol was less efficient (about 50% reduction at 1.0 mu/ml). In HepG2 cells linalool and eucalyptol reduced DNA damage by 30% and 40%, respectively, while myrcene was ineffective. The repair of t-BOOH induced DNA damage, studied in HepG2 cells, was not affected by monoterpenes. The results indicate that linalool, eucalyptol and myrcene have substantial protective effect against oxidant induced genotoxicity, which is predominately mediated by their radical scavenging activity. (C) 2008 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据