期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 47, 期 7, 页码 1610-1617出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2009.04.009
关键词
Anemarsaponin B; Cyclooxygenase-2; Inducible nitric oxide synthase; Nuclear factor-kappa B; p38 MAP kinase
资金
- Korea Science and Engineering Foundation [RI 3-200-2-020-010020]
- Seoul Research and Business Development Program [10524]
Anemarrhena asphodeloides is widely used in traditional Chinese medicine, and is known to have anti-diabetic and diuretic effects. In this study, we evaluated the anti-inflammatory effects of anemarsaponin B (ASB), a steroidal saponin isolated from the rhizomes of A. asphodeloides (Liliaceae), in LPS-stimulated RAW 264.7 macrophage cell line. ASB significantly and dose-dependently decreased the protein and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). ASB also reduced the expressions and productions of pro-inflammatory cytokines, including those of tumor necrosis factor-alpha TNF-alpha) and interleukin-6 (IL-6). Electrophoretic mobility shift assay (EMSA) and reporter gene assays revealed that ASB attenuated the LPS-induced DNA binding and transcriptional activity of nuclear factor-kappa B (NF-kappa B). In addition, it was found that pretreatment with ASB significantly inhibited the nuclear translocation of the p65 subunit of NF-kappa B by blocking the phosphorylation of inhibitory kappa B-alpha (I kappa B alpha). On the other hand, ASB inhibited the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3), which are both involved in the p38 pathway. Taken together, these results suggest that anti-inflammatory effect of ASB in LPS-treated RAW 264.7 macrophages is associated with the inhibition of NF-kappa B transcriptional activity, possibly via the p38 MAP kinase pathway. (C) 2009 Published by Elsevier Ltd.
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