期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 46, 期 4, 页码 1389-1397出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2007.09.003
关键词
carob fibre; Ceratonia siliqua L.; DNA-synthesis; gallic acid; hydrogen peroxide; proliferation
An extract of the Mediterranean carob (Ceratonia siliqua L.) pod (carob fibre extract), products formed after its fermentation by the gut flora and the major phenolic ingredient gallic acid (GA), were comparatively investigated for their influence on survival and growth parameters of colon adenocarcinoma HT29 cells and adenoma LT97 cells. Hydrogen peroxide (H2O2) formation in the cell culture media was quantified. After 1 h 97 +/- 4 mu M or 70 +/- 15 mu M were found in HT29 medium and 6 +/- 1 mu M or 3 +/- 3 mu M in LT97 medium for carob fibre extract or GA, respectively. After 72 h carob fibre extract reduced survival of rapidly proliferating HT29 cells (by 76.4 +/- 12.9%) whereas metabolic activity and DNA-synthesis were only transiently impaired. Survival of slower growing LT97 cells was less decreased (by 21.5 +/- 12.9%), but there were marked effects on DNA-synthesis (reduction by 95.6 +/- 7%, 72 h). GA and fermented carob fibre did not have comparable effects. Thus, carob fibre extract resulted in H2O2 formation, which, however, could not explain impairment of cell growth. The differently modulated growth of human colon cell lines was more related to proliferation rates and impairment of DNA-synthesis than to H2O2 formation. (c) 2007 Elsevier Ltd. All rights reserved.
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