Analysis of urinary andrographolides and antioxidant status after oral administration of Andrographis paniculata leaf extract in rats

A simple high-performance liquid chromatography (HPLC) method was developed to determine the content of andrographolide (AP) and 14-deoxy-11, 12-dideoxyandrographolide (DIAP) in a pooled urine of rat obtained within 24 h after an oral dose of Andrographis paniculata leaf extract at 1 g/kg body weight. Cumulative urinary excretion of AP and DIAP in 24 h after oral administration of the extract was 0.88% and 1.61% of oral dose administered, respectively. The extract showed significant reduction (p < 0.05) of MDA levels and elevation of total antioxidant status in rat urine samples collected in 24 after oral administration. (C) 2008 Elsevier Ltd. All rights reserved.