期刊
FOOD AND BIOPRODUCTS PROCESSING
卷 92, 期 C4, 页码 383-392出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.fbp.2013.08.014
关键词
Enzyme immobilization; Packed bed reactor; Batch reactor; beta-galactosidase; Biolacta N5; Eupergit C 250L
资金
- European Union
- European Regional Development Fund
- The Ministry of Economic Affairs, Agriculture and Innovation
- Peaks in the Delta
- Municipality of Groningen
- Province of Groningen
- Province of Fryslan
- Province of Drenthe
- Dutch Carbohydrate Competence Center [CCC WP9]
We report here that the usage of immobilized enzyme in a continuous packed bed reactor (PBR) can be a good alternative for GOS production instead of the traditional use of free enzyme in a. batch reactor. The carbohydrate composition of the product of the PBR with immobilized enzyme was comparable to that of the batch reactor with free enzyme. The stability of the immobilized enzyme at a lactose concentration of 38% (w/v) and at 50 C was very high: the half-life time of the immobilized enzyme was approximately 90 days. The enzymatic productivity of GOS production using immobilized enzyme in a PBR can be more than six times higher than that of GOS production with free enzyme in a batch reactor. Besides, when aiming for an equal volumetric productivity to the batch process in designing a PBR, the volume of the PBR can be much smaller than that of the batch reactor, depending on the enzyme dosage and the run time of a single batch. (C) 2013 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
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