Liquid-Liquid Extraction of Lipase Using Aqueous Two-Phase System

The application of aqueous two-phase extraction for the downstream processing of lipase has been exploited. The influence of system parameters such as phase forming salts, molecular weight of the phase forming polymer, system pH, tie line length, and phase volume ratio on the partitioning behavior of lipase was evaluated. The aqueous two-phase system consisting of PEG6000 and disodium phosphate (Na2HPO4) resulted in one-sided partitioning of lipase with partition coefficient 0.11, activity recovery 116%, and purification factor of 2.25. Further, the purity of lipase was increased to 3.59-fold using multi-stage extractions.