期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 26, 期 3, 页码 410-419出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2014.938319
关键词
hapten; cucumber; innovative method; chlorothalonil; monoclonal antibody; sensitivity
资金
- Key Programmes from MOST [2012AA06A303]
- Jiangsu Province
- MOF [201310128]
A sensitive monoclonal antibody for chlorothalonil was created based on a new hapten, which was prepared by an innovative method described in this paper. The hapten was generated by the reaction of original chlorothalonil drugs with 6-aminocaproic acid, distinct from that using glycol and ethylenediamine reported in other papers. A cell strain, (No. 4D7), was obtained with IC50 0.36 ng/mL, which was the best sensitivity obtained to date. The good recovery obtained in tests for cucumber indicated that the method possesses excellent accuracy and sensitivity. These results show that the developed enzyme-linked immune technology based on 4D7 is a very useful analytical tool for the detection of chlorothalonil residues and is important to immunity studies on chlorothalonil.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据