期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 26, 期 3, 页码 356-365出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2014.914891
关键词
semicarbazide; nitrofurazone; monoclonal antibody; indirect competitive enzyme-linked immunosorbent assay
资金
- Key Programs from MOST [2012AA06A303, 2012BAD29B04]
- Jiangsu Province
- MOF
- MOE [201310135, JUSRP51308A]
A highly sensitive and specific monoclonal antibody was prepared to detect semicarbazide (SEM). Hapten 4-{[(aminocarbonyl)hydrazono]methyl}benzoic acid (4-CPSEM) was synthesised through the condensation reactions of SEM and 4-carboxybenzaldehyde (4-CBA). The active ester method was employed to couple the hapten to bovine serum albumin, keyhole limpet hemocyanin and ovalbumin (OVA) to obtain conjugates of immunogens and coating antigens. A novel hapten 2-[(aminocarbonyl)hydrazono]acetic acid (SEM-A) was also synthesised from SEM and oxoacetic acid to prepare a heterologous coating antigen (SEM-A-OVA). The linear ranges of inhibition curves in the optimised indirect competitive enzyme-linked immunosorbent assay (icELISA) method were in the range of 0.0071-0.056 ng mL(-1) and 0.018-0.209 ng mL(-1) for coating antigens SEM-A-OVA and 4-CPSEM-OVA, respectively. The IC50 was 0.019 ng mL(-1) for SEM (SEM in the form of 4-nitrobenzaldehyde semicarbazone) and 0.13 ng mL(-1) for 2-nitrobenzaldehyde semicarbazone, both of which were well below the minimum required performance limit of 1 mu g center dot kg(-1) set by the European Commission.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据