期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 25, 期 3, 页码 322-331出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2013.791970
关键词
polyclonal antibody; immunogen; ELISA; salbutamol
Salbutamol is a beta-agonist which is strictly prohibited in food production. Designing a rapid immunoassay detection method is essential for effective supervision, and synthesis of antigen is the key to successful antibody production. In this study, a new method of synthesising immunogen and coating antigen for ELISA was established, using methanal as a linker to connect salbutamol sulphate with the carrier proteins bovine serum albumin and ovalbumin. The conjugates were identified through UV spectrophotometry and SDS-PAGE, and the amino groups in proteins were measured using 2,4,6,-trinitrobenzenesulphonic acid. Immunisation of rabbits with immunogen produced a polyclonal antibody with high sensitivity and specificity. Indirect and competitive indirect ELISAs were used to determine antibody titre and IC50, which were 1,280,000 and 0.736 ng mL(-1), respectively.
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