期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 24, 期 2, 页码 165-177出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2012.670615
关键词
dipropyl phthalate; ELISA; polyclonal antibody; antigen; plastic food contact materials
资金
- National Natural Science Foundation of China [21075002, 21175004]
- Key Program of the Natural Science Foundation of the Anhui Higher Education Institutions of China [KJ2011A141]
A highly sensitive and specific direct competitive enzyme-linked immunosorbent assay has been developed for the detection of dipropyl phthalate (DPrP) based on antibody-coating format. The specific polyclonal antibodies against DPrP raised in rabbits. The conjugates of the antigen with horseradish peroxidase (HRP) were used as the detectable probe. Under the optimal conditions, the limit of detection of the method was 0.01 ng/mL, and a good quantitative working range of 0.0116 ng/mL (R 2=0.995) was obtained. The cross-reactivities of four structurally related phthalate esters were below 12% and did not interfere significantly the analysis of DPrP. The accuracy of the immunoassay has been evaluated and validated by analyzing real and spiked leachate from plastic food contact materials. The recoveries of DPrP ranged from 85.9% to 109.4%. This developed dc-ELISA was reliable, accurate and highly sensitive.
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