期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 23, 期 3, 页码 265-272出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2011.617358
关键词
PNA; food allergen; polyclonal antibody; sandwich ELISA
资金
- National Natural Foundation of China [21071066, 20835006, 91027038, 21101079, 21175034]
- MOST [2010AA06Z302, 2010DFB3047, 2008BAK41B03, 2009BAK61B04, 2008ZX08012-001, 2010GB2C100167]
- Natural Science Foundation of Jiangsu Province
- MOF
- MOE [BK2010001, BK2010141, JUSRP10921, JUSRP11019, 201110060, 201110016, 201110061, 201010078, 201010216]
In this study, two anti-peanut agglutinin (PNA) polyclonal antibodies were successfully prepared. Using mouse anti-PNA polyclonal antibody as the capture antibody and rabbit anti-PNA polyclonal antibody as the detection antibody, a novel sandwich enzyme-linked immunosorbent assay (ELISA) was developed to quantify PNA. The detection limit of the ELISA method was low (0.49 ng/mL), and the linear dynamic range was between 0.78 and 100 ng/mL. The recovery ranged from 93.86% to 139.3%, whereas the intra-and inter-assay coefficients of variation were less than 10.25% and 12.06%, respectively. Sample analysis verified this method as a reliable tool for the detection of PNA in processed foods.
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