期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 21, 期 3, 页码 209-218出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540101003596644
关键词
glycinin; beta-conglycinin; enterocyte; apoptosis; proliferation; migration; pigs
资金
- National Natural Science Foundation of P.R. China [30430520]
Glycinin and beta-conglycinin have been identified as major food/feed allergens. But effects of glycinin and beta-conglycinin on enterocyte migration in piglets are scare. Fifteen weanling (7.06 +/- 0.18 kg) General No. 1 barrows, weaned at 28 days, were used. The piglets were randomly allotted to three (A, B and C) treatments with five replicates. The piglets in the A group (control group) were fed diets without ingredients originating from leguminous products, while the piglets in the B or C groups were fed the diets containing purified glycinin or beta-conglycinin, which replaced protein in Group A by 4%. All the experimental periods were followed for 7 days. Five-micrometre thick sections of small intestinal tissue were stained with the TUNEL method to assess apoptotic activity, and with Ki-67 immunohistochemistry to assess cellular proliferation. The results indicated that glycinin or beta-conglycinin increased proliferative index and apoptotic index in duodenum for piglets (P<0.05).
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