期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 21, 期 2, 页码 143-156出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540100903452122
关键词
antigenicity; -lactoglobulin; whey protein isolate; glucose; Maillard reaction
资金
- National Natural Science Foundation of China [30471224, 30871817]
- National Science and Technology Ministry of China [2006BAD27B04, 2006BAD04A06]
The effect of weight ratio of protein to sugar (0.17-7.83), temperature (40-60 degrees C) and time (24-120 h) on the antigenicity of -lactoglobulin (-LG) in conjugates of whey protein isolate (WPI) with glucose was investigated. Response surface methodology was used to carry out an optimisation of the reaction conditions leading to the minimum antigenicity of -LG. Conjugation of WPI with glucose could reduce effectively the antigenicity of -LG. This reduction of antigenicity could be controlled by regulating three independent variables (weight ratio of WPI to glucose, temperature and time). The model for optimal reaction conditions of a lower antigenicity of -LG was established. Weight ratio of WPI to glucose had the greatest effect on the antigenicity of -LG. Reaction time influenced the antigenicity of -LG to a lesser extent.
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