期刊
FOOD ANALYTICAL METHODS
卷 6, 期 1, 页码 28-35出版社
SPRINGER
DOI: 10.1007/s12161-012-9413-7
关键词
Synthetic phenolic antioxidants; High-performance liquid chromatography; Cloud point extraction; Tergitol TMN-6; Ultrasound-assist
资金
- Analysis Test Research Center of Kunming University of Science and Technology, Yunnan Province, China
- Innovation Fund for Small and Medium Technology Based Firms [10C26215305131]
A cloud-point extraction (CPE) method using Tergitol TMN-6 (TMN-6) non-ionic surfactant was developed for the extraction and preconcentration of propyl gallate (PG), tertiary butyl hydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) in edible oils. The optimum conditions of CPE were 1.5 % (v/v) Tergitol TMN-6, 1 % (w/v) NaCl, ultrasound-assist 15 min at 49 KHz, 20 min equilibrated at 45 A degrees C, and centrifugation for 10 min at 3,000 rpm. The surfactant-rich phase was then analyzed by reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet detection at 280 nm, under gradient separation, using methanol and 1.5 % (v/v) acetic acid. Under the study conditions, four synthetic phenolic antioxidants (SPAs) were successfully separated within 24 min. Limits of detection in the studied edible oils were in the range of 1.6 to 9.0 ng mL(-1). The high recoveries of the spiked edible oils were obtained in the range 90-98 %. The CPE method has been shown to be a potentially useful methodology for the preconcentration of the target analytes, with a preconcentration factor of 25. This method was compared with cloud point extraction (using Triton X-114) and liquid-liquid extraction (using methanol).
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