Determination of the exposure parameters that maximise the concentrations of the anaesthetic/sedative eugenol in rainbow trout (Oncorhynchus mykiss) skin-on fillet tissue

Studies were conducted to determine the anaesthetic/sedative concentrations and durations that would maximise anaesthetic/sedative residue concentrations in rainbow trout (Oncorhynchus mykiss) skin-on fillet tissue. Rainbow trout (167-404 g) were exposed to 50 mg l(-1) AQUI-S (R) 20E (10% active ingredient, eugenol) in 17 degrees C freshwater for durations up to 1440 min, 100 and 250 mg l(-1) AQUI-S (R) 20E for durations up to 240 min, and 500 and 1000 mg l(-1) AQUI-S (R) 20E for durations up to 90 min. Fish exposed to 100 mg l(-1) AQUI-S (R) 20E for durations of 30, 60, 120 and 240 min had the greatest eugenol concentrations in the fillet tissue, 50, 58, 54 and 62 mu g g(-1), respectively. All other exposure concentrations and durations resulted in significantly lower eugenol concentrations, i. e. all < 39 mu g g(-1).