Determination of neotame in beverages, cakes and preserved fruits by column-switching high-performance liquid chromatography

A column-switching HPLC method for the determination of neotame in beverages, cakes and preserved fruits was developed. After pre-treatment using a Waters Oasis HLB cartridge, the sample solution was separated on two C18 columns using a column-switching technique with a six-port valve. UV detection was performed at 210 nm. The effects of eluent composition and eluate volume on the retention and elution of neotame on SPE cartridge were investigated. The method is simple, rapid, sensitive and has good reproducibility. RSD was lower than 5% (n = 5). The calibration curve of neotame was in the range 5-100 mu g/ml with good linearity (r2 = 0.999). Because neotame was concentrated 10 times from an original sample to a sample solution by solid-phase extraction (SPE), the limit of quantification (LOQ) of the method was 0.5 mg/kg. The recovery yields of neotame spiked in foods was 92% with a coefficient of variation 3.2%. The proposed column-switching HPLC method can be successfully used to determine neotame in routine inspection work.