期刊
FLY
卷 6, 期 2, 页码 75-79出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/fly.19553
关键词
Drosophila melanogaster; Galleria mellonella; entomopathogenic nematodes; Steinernema feltiae; Heterorhabditis bacteriophora
资金
- Swedish Research Council [VR-NT 2010-5118]
- Cancerfonden [CAN 2010/553]
- Swedish Foundation for International Cooperation in Research and Higher Education (STINT)
- Carl-Tryggers Foundation
- Grant Agency of the Czech Republic [GA206/09/P470]
The infective juveniles (IJs) of entomopathogenic nematodes (EPNs) seek out host insects and release their symbiotic bacteria into their body cavity causing septicaemia, which eventually leads to host death. The interaction between EPNs and their hosts are only partially understood, in particular the host immune responses appears to involve pathways other than phagocytosis and the canonical transcriptional induction pathways. These pathways are genetically tractable and include for example clotting factors and lipid mediators. The aim of this study was to optimize the nematode infections in Drosophila melanogaster larvae, a well-studied and genetically tractable model organism. Here we show that two nematode species namely Steinernema feltiae and Heterorhabditis bacteriophora display different infectivity toward Drosophila larvae with the latter being less pathogenic. The effects of supporting media and IJ dosage on the mortality of the hosts were assessed and optimized. Using optimum conditions, a faster and efficient setup for nematode infections was developed. This newly established infection model in Drosophila larvae will be applicable in large scale screens aimed at identifying novel genes/pathways involved in innate immune responses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据