期刊
FLY
卷 5, 期 3, 页码 242-246出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/fly.5.3.15837
关键词
morphogen gradient; Bicoid; half-life; diffusion constant; steady state; positional information; robustness; Drosophila; fates-shifted; ubiquitination
资金
- NIH
- NSF
- AHA
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [0843424] Funding Source: National Science Foundation
In a recent publication, 1 we identified a novel F-box protein, encoded by fates-shifted (fsd), that plays a role in targeting Bcd for ubiquitination and degradation. Our analysis of mutant Drosophila embryos suggests that Bcd protein degradation is important for proper gradient formation and developmental fate specification. Here we describe further experiments that lead to an estimate of Bcd half-life, <15 min, in embryos during the time of gradient formation. We use our findings to evaluate different models of Bcd gradient formation. With this new estimate, we simulate the Bcd gradient formation process in our own biologically realistic 2-D model. Finally, we discuss the role of Bcd-encoded positional information in controlling the positioning and precision of developmental decisions.
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