期刊
FITOTERAPIA
卷 92, 期 -, 页码 72-78出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.fitote.2013.10.008
关键词
Bletilla striata; Glucomannan; Structure; Anti-fibrosis; TGF-beta(1)
资金
- National Natural Science Foundation [81173527/H2803, 81202900/H2803]
- National Key Technologies R&D Program of China [2011ZX09101-009-02]
- State Key Laboratory of Natural Medicines, China Pharmaceutical University [SKLNMKF201207]
A novel water-soluble polysaccharide, designated Bletilla striata polysaccharide b (BSPb), was isolated from a traditional Chinese medicinal herb, Bletilla striate. The crude polysaccharide was obtained by hot water extraction and purified by ion-exchange chromatography. The molecular weight was 260 kDa as determined by HPGPC (high performance gel permeation chromatography). Monosaccharide analysis showed that BSPb contained glucose and mannose with a molar ratio of 3:1. Methylation and spectroscopic analyses with 1D and 2D NMR spectroscopy showed that the backbone of BSPb is mainly composed of (1 -> 2)-linked alpha-D-mannopyranose and (1 -> 4)-linked beta-D-glucopyranose residues. In this study, BSPb plays an important role in protection against the renal fibrosis effect, which is probably mediated by down-regulated TGF-beta RI, TGF-beta RII, and alpha-SMA in vitro. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据