期刊
FITOTERAPIA
卷 90, 期 -, 页码 140-150出版社
ELSEVIER
DOI: 10.1016/j.fitote.2013.07.015
关键词
Mesua ferrea; Coumarins; Staphylococcus aureus; Clinical strain; NorA-pump inhibitor
资金
- IMTECH
- NIPER
The purpose of this investigation was to study the modulator and efflux pump inhibitor activity of coumarins isolated from Mesua ferrea against clinical strains as well as NorA-over expressed strain of Staphylococcus aureus 1199B. Seven coumarins were tested for modulator activity using ethidium bromide (EtBr) as a substrate. Compounds 1, 4-7 modulated the MIC of EtBr by >= 2 fold against wild type clinical strains of S. aureus 1199 and S. aureus 1199B, whereas compounds 4-7 modulated the MIC of EtBr by >= 16 fold against MRSA 831. Compounds 1,4-7 also reduced the MIC of norfloxacin by >= 8 fold against S. aureus 1199B, and 4-6 reduced the MIC of norfloxacin by >= 8 fold against MRSA 831 at half of their MICs. Inhibition of EtBr efflux by NorA-overproducing S. aureus 1199B and MRSA 831 confirmed the role of compounds 4-6 as NorA efflux pump inhibitors (EPI). Dose-dependent activity at sub-inhibitory concentration (6.25 mu g/mL) suggested that compounds 4 and 5 are promising EPI compared to verapamil against 1199B and MRSA 831 strains. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据