期刊
FITOTERAPIA
卷 82, 期 4, 页码 595-600出版社
ELSEVIER
DOI: 10.1016/j.fitote.2011.01.010
关键词
Abelmoschus manihot flowers; Flavonoids; 3T3-L1; TG; FFA; mRNA expression
资金
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry [[2010] 609]
- Applied Basic and Advanced Research Program of Tianjin [09JCYBJC16300, 10SYSYJC28900]
- Ministry of Science and Technology of China [2009ZX09311-002]
- Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)
The 95% EtOH extract from the flowers of Abelmoschus manihot (L) Medic showed inhibitory activity on TG accumulation in 3T3-L1 preadipocyte. Chemical studies on the active fraction led to the isolation of 14 flavonoids (1-14). To clarify the multi-mechanism of the isolates on preadipocyte differentiation, the levels of TG and FFA and the related role transcription factors (PPAR gamma, CEBP/alpha, and ap2) expression were evaluated. At the concentration of 30 mu M, compounds 1-6 and 10-14 showed inhibitory activity on TG accumulation significantly in mature 3T3-L1 cells. 1, 2, 4-7, 9, 10, 13, and 14 reduced the level of FFA. At the molecular level, the mRNA expressions of PPAR gamma, CEBP/alpha, and ap2 were down-regulated by compounds 1, 5, 9, 12, 13; 1-8, 10-14; and 1-4, 6, 8-12, 14, respectively. The structure-activity relationships of the 14 flavonoids were also discussed. (C) 2011 Published by Elsevier B.V.
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