期刊
FITOTERAPIA
卷 81, 期 8, 页码 1006-1012出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.fitote.2010.06.020
关键词
Macleaya cordata; Isoquinoline alkaloids; Phenolic acids; Fatty acids; Sanguinarine reductase; Antimicrobial activity
资金
- Grant Agency of the MSMT [MSM 6198959216]
- Grant Agency of the Czech Republic [GA CR 525/07/0871, GA CR 525/08/0819]
- AV CR-DAAD SRN [D21-CZ15/07-08]
Macleaya cordata (plume poppy) is a source of bioactive compounds, mainly isoquinoline alkaloids which are used in phytopreparations with anti-inflammatory and antimicrobial activities. In this study, the alkaloids sanguinarine, chelerythrine, their dihydro derivatives, protopine and allocryptopine and phenolics, gallic, protocatechuic, p-hydroxybenzoic, m-hydroxybenzoic, gentisic, p-coumaric, caffeic, ferulic and sinapic acids were determined in extracts prepared from M. cordata aerial part, seeds, and seed capsules using HPLC with UV detection and/or LC/MS with electrospray ionization. The highest content of sanguinarine and chelerythrine was found in capsules. Protopine and allocryptopine were major alkaloids in leaves including footstalks. The seed oil contained dihydrosanguinarine, dihydrochelerythrine and twelve fatty acids of which linoleic, oleic, palmitic and stearic acids predominated. In addition, sanguinarine reductase, a key enzyme in sanguinarine/dihydrosanguinarine equilibrium in plants, was found for the first time, in the soluble proteins of leaves. Finally, extracts were tested for antimicrobial activity using the microdilution method on standard reference bacterial strains. (C) 2010 Elsevier B.V. All rights reserved.
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