Structure analysis of water-soluble polysaccharide CPPS3 isolated from Codonopsis pilosula

Codonopsis pilosula is a perennial species of flowering plants propagated in Northeast Asia A water-soluble polysaccharide. CPPS3, was extracted from the root of Codonopsis pilosula by boiled water extraction and ethanol precipitation The molecular weight was estimated to be 7 4 x 10(4) Da determined by using Gel permeation chromatography Monosaccharide composition and the structure of the polysaccharide were determined by gas spectroscopy, Fourier transform IR (FT-IR) spectroscopy, NMR spectroscopy and mass spectroscopy and some chemical method analysis was made The components were galactose. arabinose and rhamnose in the molar ratio of 1 13 1 12 1 The main chain of CPPS3 is illustrated to be (1 -> 3)-linked-beta-GalpNAc, (1 -> 3)-linked-alpha-Rhap and (1 -> 2,3)-beta-Galp (C) 2009 Elsevier B V All rights reserved.