Molecular characterization of Desmodium species - An important ingredient of 'Dashmoola' by RAPD analysis

Identification of medicinal plants by their molecular signature is a fast growing tool. The identification of Desmodium gangeticum (L.) DC. (Shalparni, a constituent of Ayurvedic formulation Dashmoolarishtha) was carried out using genomic approach. Authentic samples of D. gangeticum(L.) DC, D. velutinum (Willd.) DC. and D. triflorum (L.) DC. were analyzed and compared to commercial samples of various origin. Within twenty primers used. eleven gave 223 RAPD fragments. RAPD profiles of three species showed very low similarity index (0.21-0.39), whereas market samples showed high similarity of 0.82-0.89 with authenticated D. gangeticum. (C) 2008 Elsevier B.V. All rights reserved.