期刊
FITOTERAPIA
卷 79, 期 1, 页码 32-36出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.fitote.2007.07.008
关键词
Rhus javanica var. roxburghiana; DNA strand scission; Flavonoids
资金
- National Science Council of the Republic of China [NSC 94-2320B037-033]
The flavonoids isolated from the stems of Rhus javanica var. roxburghiana, taxifolin (1), fisetin (2), fustin (3), 3,7,4'-trihydroxyflavanone (4) and 3,7,4'-trihydroxyflavone (5) caused breakage of supercoiled plasmid pBR322 DNA in the presence of Cu(II). Cu(1) was shown to be an essential intermediate by using the Cu(I)-specific sequestering reagent neocuproine. The Cu(II)-mediated DNA scissions induced by 1, 2, 3 and 5 were inhibited by the addition of catalase and exhibited DNA strand break by the addition of KI and superoxide dimutase (SOD), while in the Cu(II)-mediated DNA scissions induced by 4 was inhibited by the addition of KI, SOD, and catalase. It is concluded that 1, 2,3, and 5 can induce H(2)O(2) and superoxide anion, while 4 can induce OH(.) and H(2)O(2) and subsequent oxidative damage of DNA in the presence of cu(II). (c) 2007 Elsevier B.V. All rights reserved.
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