期刊
FISHERIES SCIENCE
卷 77, 期 1, 页码 151-157出版社
SPRINGER JAPAN KK
DOI: 10.1007/s12562-010-0306-7
关键词
Funazushi; Bacterial community; PCR-DGGE; Lactobacillus plantarum; L. acetotolerans
类别
资金
- Ministry of Education, Science, Sports and Culture of Japan [B 16380143]
Funazushi (fermented Crucian Carp with rice) is a fermented fish product found only around Lake Biwa in Shiga Prefecture, Japan. It is characterized by a unique cheese-like flavor and characteristic sour taste. We analyzed the changes in the microbial community during funazushi fermentation by denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA fragments (PCR-DGGE) and by plate counts. The plate counts showed that lactic acid bacteria reached 8.0 log(10) CFU/g within 7 days of fermentation initiation before decreasing slowly to 4.0 log(10) CFU/g during the remainder of 1-year study period. PCR-DGGE revealed that the dominant bacteria in the initial (days 14 and 30) and latter (days 90, 180, and 360) periods of fermentation were Lactobacillus plantarum and L. acetotolerans. This is the first identification of L. acetotolerans in funazushi as traditional cultivation techniques have not been sufficiently sensitive. This is the first report of PCR-DGGE being used to assess the microbial community in funazushi. This technique was also found to be effective in profiling microbial diversity.
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