期刊
FISH PHYSIOLOGY AND BIOCHEMISTRY
卷 40, 期 1, 页码 93-104出版社
SPRINGER
DOI: 10.1007/s10695-013-9827-1
关键词
Amino acid transporter y(+)LAT1; Cloning; Fasting; Grass carp; mRNA expression; Tissue distribution
资金
- Huazhong Agricultural University Scientific & Technological Self-innovation Foundation [2009QC022]
The solute carrier family 7A, member 7 gene encodes the light chain- y(+)L amino acid transporter-1 (y(+)LAT1) of the heterodimeric carrier responsible for cationic amino acid (CAA) transport across the basolateral membranes of epithelial cells in intestine and kidney. Rising attention has been given to y(+)LAT1 involved in CAA metabolic pathways and growth control. The molecular characterization and function analysis of y(+)LAT1 in grass carp (Ctenopharyngodon idellus) is currently unknown. In the present study, full-length cDNA (2,688 bp), which encodes y(+)LAT1 and contains a 5'-untranslated region (319 bp), an open reading frame (1,506 bp) and a 3'-untranslated region (863 bp), has been cloned from grass carp. Amino acid sequence of grass carp y(+)LAT1 contains 11 transmembrane domains and shows 95 %, 80 % and 75 % sequence similarity to zebra fish, amphibian and mammalian y(+)LAT1, respectively. The tissue distribution and expression regulation by fasting of y(+)LAT1 mRNA were analyzed using real-time PCR. Our results showed that y(+)LAT1 mRNA was highly expressed in midgut, foregut and spleen while weakly expressed in hindgut, kidney, gill, brain, heart, liver and muscle. Nutritional status significantly influenced y(+)LAT1 mRNA expression in fish tissues, such as down-regulation of y(+)LAT1 mRNA expression after fasting (14 days).
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