期刊
FISH PHYSIOLOGY AND BIOCHEMISTRY
卷 35, 期 3, 页码 501-509出版社
SPRINGER
DOI: 10.1007/s10695-008-9287-1
关键词
Hyperosmotic stress; Growth; Osmoregulation; Semi-quantitative RT-PCR
资金
- Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul [01/1602.5]
- Universidade Federal do Rio Grande (FURG, Brazil)
- Programa de Pos-Graduacao em Aquicultura (PPGAq-FURG).
Growth hormone (GH) action is the result of an intracellular cascade initiated just after its interaction with the growth hormone receptor (GHR) located on the surface of target cells. This cascade culminates with the transcription of target genes, such as the insulin-like growth factors (IGFs), which are responsible for most GH biological effects. In addition to its central role in growth, fish GH is also involved with osmoregulatory control. Within this context, the objective of the present work was to isolate GH, GHR, and IGF-I cDNAs from the Brazilian flounder Paralichthys orbignyanus and evaluate whether these genes are induced by hyperosmotic stress. The obtained results indicated that GH mRNA had a significant peak only 24 h after hyperosmotic stress. In gills, GHR mRNA was significantly increased after 7 days. In liver, GHR and IGF-I mRNAs were significantly increased in 72 h and both reached even higher levels after 7 days. These results indicate that hyperosmotic stress can increase GH sensitivity in the gills and liver of P. orbignyanus and, consequently, improve IGF-I production. The management of this parameter could be useful in achieving better growth performance for this and other commercially important species in which GH has a direct correlation with osmoregulatory mechanisms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据