期刊
FISH & SHELLFISH IMMUNOLOGY
卷 41, 期 2, 页码 608-617出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2014.10.014
关键词
Litopenaeus vannamei; Toll signal pathway; LvCactin; Antimicrobial peptide genes; RNA interference
资金
- National Basic Research Program [2014CB138600]
- National Natural Science Foundation of China [NSFC 30871928]
- Special Fund for Agro-scientific Research in the Public Interest [201003020]
- Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
Cactin (Cactus interactor) is a conserved protein which was initially discovered as a novel interactor of Drosophila IkB protein Cactus. Cactin was always characterized as a negative regulator of many different developmental processes, but only found to play an immune role in humans. To better know the immune function of Cactin gene, Litopenaeus vannamei Cactin (LvCactin) was identified and characterized in this study. The full-length cDNA of LvCactin is 3, 150 bp long, with an open reading frame (ORF) encoding a Cactin_mid domain in the N-terminus 356-547 residues and a CactinC_cactus domain in the C-terminal 731-855 residues. The LvCactin protein was located in the cytoplasm and LvCactin mRNA was constitutively expressed in healthy L vannamei, with the highest expression level in the eyestalk. LvCactin could be regulated in hemocytes after lipopolysaccharide (LPS), poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV) challenges. Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvCactin inhibited the promoters of Drosophila and shrimp antimicrobial peptide (AMP) genes. Knockdown of LvCactin by RNA interference (RNAi) increased the expression of shrimp AMP genes PEN4, crustin and ALF2 but not Lyz2. However, the mortality rates of LvCactin-knockdown shrimp in response to V. parahaemolyticus or WSSV infections were not significantly different from those of the control group. Taken together, all the results suggested that LvCactin may play a role in innate immune in L vannamei. (C) 2014 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据