4.7 Article

Differential regulation of cathelicidin in salmon and cod

期刊

FISH & SHELLFISH IMMUNOLOGY
卷 35, 期 2, 页码 532-538

出版社

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2013.05.005

关键词

Innate immunity; Cell culture; Gene expression; Cell signalling; Cathelicidin

资金

  1. Icelandic Centre for Research (RANNIS)
  2. University of Iceland Research Fund
  3. University of Iceland Eggert V. Briem Fund

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Antimicrobial peptides (AMPs) are an important component of innate immunity in vertebrates. The cathelicidin family of AMPs is well characterized in mammals and has also been reported in several fish species. In this study we investigated the regulation of cathelicidin expression in a gadoid and a salmonid cell-line in order to dissect the signalling pathways involved. For this, fish cells were treated with microbial lysates, purified microbial components and commercial signalling inhibitors and expression of cathelicidin was assessed with quantitative real-time PCR (qPCR). We found that cathelicidin expression was induced in both cell lines in response to microbial stimuli, but the response patterns differed in these evolutionary distant fish species. Our data suggest that in salmonids, pattern recognition receptors such as TLR5 may be involved in the stimulation of cathelicidin expression and that the signalling cascade can include PI3-kinase and cellular trafficking compartments. A detailed knowledge of the regulating factors involved in AMP-related defence responses, including cathelicidin, could help in developing strategies to enhance the immune defence of fish. (C) 2013 Elsevier Ltd. All rights reserved.

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