期刊
FISH & SHELLFISH IMMUNOLOGY
卷 34, 期 2, 页码 623-631出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2012.12.001
关键词
C-reactive protein; Pentraxin; Cynoglossus semilaevis; Phagocytosis
资金
- National Natural Science Foundation of China [31025030]
- National Basic Research Program of China [2012CB114406]
- Chinese Academy of Sciences [KZCX2-EW-Q213, KSCX2-EW-G-12B]
- Taishan Scholar Program of Shandong Province
Pentraxins are a family of evolutionarily conserved proteins that play an important part in innate immunity. C-reactive protein (CRP) is a member of the pentraxin family and in humans is known to be the major acute phase protein. In this work, we report the identification and analysis of a CRP, CsCRP, from half-smooth tongue sole (Cynoglossus semilaevis). CsCRP is composed of 228 amino acid residues and possesses a Pentraxin/CRP domain. Expression of CsCRP occurred in a wide range of tissues and was upregulated by pathogen infection in kidney, spleen, blood, and, in particular, liver. Following bacterial infection, CsCRP level in blood rose rapidly within 12 h and was approximately 3.8 fold of that of the basal level. Purified recombinant CsCRP (rCsCRP) was able to interact with Gram-negative and Gram-positive bacteria including those of pathogenic nature in a dose-dependent manner. When peripheral blood leukocytes (PBL) were infected with bacterial pathogen in the presence of rCsCRP, the respiratory burst and phagocytic capacity of the cells were increased to significant extents. Taken together, these results indicate that CsCRP is an acute phase protein that plays a role in innate immune defense against bacterial infection. (C) 2012 Elsevier Ltd. All rights reserved.
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