期刊
FISH & SHELLFISH IMMUNOLOGY
卷 32, 期 1, 页码 26-34出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2011.10.010
关键词
Anti-lipopolysaccharide factor; Vibrio harveyi; White spot syndrome virus Penaeus; monodon; Gene silencing
资金
- Thailand National Center for Genetic Engineering and Biotechnology (BIO-TEC)
- Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission [FW643A]
- Chulalongkorn University under the Ratchadaphisek Somphot Endowment
- Faculty of Science, Chulalongkorn University [RESA1B1-11]
- Thailand Research Fund (TRF)
- Office of the Higher Education Commission
- Kasetsart University [MRG5380020]
- Thai Government Stimulus Package 2 [TKK2555]
Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides previously identified in various crustaceans. Out of five isoforms identified in Penaeus monodon, ALFPm3 is the best characterized, exhibits antibacterial and antifungal activities and can protect the shrimp from viral infections. Herein, the most recent identified ALFPm, called ALFPm6, is characterized for its potential role in the shrimp's immunity. RNA interference-mediated gene silencing was used to study the function of ALFPm6 in comparison to ALFPm3. Knockdown of ALFPm3 gene led to rapid death with a cumulative shrimp mortality of 86% within 7 days, accompanied by a 12- and 50-fold higher bacterial count after 2 days in the haemolymph and hepatopancreas, respectively, compared to the control shrimp injected with GFP dsRNA. In contrast, gene silencing of ALFPm6 alone had no effect on the shrimp mortality, but led to a significant increase in the cumulative mortality and a faster mortality rate following Vibrio harveyi and white spot syndrome virus (WSSV) infections, respectively. These results support the roles of ALFPm6 and ALFPm3 in the protection of shrimp against microbial infections. (C) 2011 Elsevier Ltd. All rights reserved.
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