期刊
FISH & SHELLFISH IMMUNOLOGY
卷 33, 期 1, 页码 92-98出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2012.04.006
关键词
Olive flounder (Paralichthys olivaceus); Testis; Antimicrobial protein; Histone H1-like protein; MEC
资金
- National Fisheries Research and Development Institute of Korea
An approximately 21 kDa antimicrobial protein was purified from an acidified testis extract of olive flounder, Paralichthys olivaceus, by ion-exchange and C-18 reversed-phase HPLC. A comparison of the N-terminal amino acid sequence with those of other known antimicrobial polypeptides revealed high homology between this antimicrobial protein and other histone H1 molecules; thus, it was designated flounder histone H1-like protein (fH1LP). fH1LP showed potent antimicrobial activity against Gram-positive bacteria, including Bacillus subtilis, Staphylococcus aureus, and Streptococcus iniae (minimal effective concentrations [MECs], 2.8-30.0 mu g/ml), Gram-negative bacteria, including Aeromonas hydrophila, Escherichia coli 031, Vibrio parahaemolyticus (MECs, 1.4-12.0 mu g/ml), and Candida albicans (MEC, 2.0 mu g/ml). cDNA cloning and tissue distribution studies of fH1LP indicated that it is constitutively expressed in testis and ovary. The fH1LP expression level was significantly dependent on developmental stage, and decreased dramatically after hatching. However, lipopolysaccharide stimulation did not induce fH1LP mRNA in other immune organs, including the kidney and spleen. These results suggest that fH1LP plays an important role in innate immunity in fish during reproduction, including mating, fertilization, and hatching. (C) 2012 Elsevier Ltd. All rights reserved.
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