期刊
FISH & SHELLFISH IMMUNOLOGY
卷 31, 期 1, 页码 148-154出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2011.02.024
关键词
CC chemokine; Miiuy croaker; Gene expression; Innate immunity
资金
- Nation Nature Science Foundation of China [31001120]
- Zhejiang Provincial Natural Science Foundation of China [Y3100013]
- Foundation of Zhejiang Educational Committee [Y200908463]
- Science and Technology Project of Zhoushan City [12048]
A CC chemokine gene was isolated from miiuy croaker (Miichthys miiuy) by expressed sequence tag analysis. The Mimi-CC cDNA contains an open reading frame of 429 nucleotides encoding 142 amino acid residues. The deduced Mimi-CC possesses the typical arrangement of four cysteines as found in other known CC chemokines (C-31, C-32, C-56, and C-70). It shares 15.3%-37.4% identity to CC chemokines of mammal and teleost. Phylogenetic analysis showed that miiuy croaker was most closely related to Atlantic cod. Genomic analysis revealed that Mimi-CC gene consists of four exons and three introns, which is not typical of CC chemokines but resembles that of CXC chemokines. Real-time quantitative RTPCR demonstrated that Mimi-CC is constitutively expressed in most tissues including lymphoid organs, and the highest expression of Mimi-CC transcripts in normal tissues was observed in muscle. Challenge of miiuy croaker with Vibrio anguillarum resulted in significant changes in the expression of CC chemokine transcripts in four tissues, especially in kidney and spleen. (C) 2011 Elsevier Ltd. All rights reserved.
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