期刊
FISH & SHELLFISH IMMUNOLOGY
卷 29, 期 5, 页码 793-802出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2010.07.010
关键词
Interferon-gamma; Classical macrophage activation; Teleost; iNOS; IL-1 beta; IL-12; CXC chemokines
资金
- Ministry of Housing, Spatial Planning and the Environment (VROM)
- [FP-6-2002-Human resources]
- [024034]
In teleost fish two IFN-gamma gene sequences were found for which two phylogenetic clusters can be distinguished. Our previous analysis of expression of these in carp led us to hypothesize that a classical IFN-gamma function is associated with the IFN-gamma 2 cluster. We investigated the evolutionary conservation of the IFN-gamma function, inducing classical activation of phagocytes, thus skewing towards a Th1-like profile of immune activation. Recombinant proteins for the carp IFN-gamma sequences of both clusters were made and we studied their effects on expression of proinflammatory mediators. Carp IFN-gamma 2, in contrast to carp IFN-gamma 1, was powerful in inducing a proinflammatory reaction in phagocytes: a classical synergistic response with lipopolysaccharide was observed for the induction of iNOS expression and NO release, for expression of CXCL9-11-like chemokines and the expression of proinflammatory cytokines IL-1 beta, TNF alpha and the IL-12 subunits p35 and p40. In contrast, like in mammals, the CXCL8-like cytokines are LPS but not IFN-gamma sensitive. These results corroborate an evolutionary conserved nature of IFN-gamma function in lower vertebrates including classical activation of phagocytes. (C) 2010 Elsevier Ltd. All rights reserved.
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