期刊
FISH & SHELLFISH IMMUNOLOGY
卷 28, 期 3, 页码 461-466出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2009.12.014
关键词
Phascolosoma esculenta; HSP70; RACE; Heavy metal; Real-time PCR
资金
- National Natural Science Foundation [40776075]
- Scientific Program of Zhejiang Province, China [2006C13089]
- Ningbo University
In this study the gene encoding HSP70 was isolated from Phascoloma esculenta by homologous cloning and rapid amplification of cDNA ends (RACE) The full-length of cDNA (2520 bp) consists of a 5'-terminal untranslated region (UTR) (125 bp), a 3'-terminal UTR (421 Lip) with a canonical polyadenylation signal sequence (AATAAA). a poly (A) tail, and an open reading frame (ORF) (1974 bp). The predicted molecular mass and isoelectric point for HSP70 is 71.6 kDa and 5 15, respectively. BLAST analysis showed that P esculenta HSP70 gene shared high similarity Classical HSP signature motifs. ATP/GTP-Binding Site Motif A. Bipartite Nuclear Targeting Sequence, the cytosolic HSP70 could be expressed in Escherichia coli BL21 After purification. the recombinant pET-HSP70 protein was used to produce the polyclonal antibody in mice and the specificity of the antibody was confirmed by Western blot analysis Fluorescent real-time quantitative PCR analysis showed that expression of Hsp70 in sipuncula was increased significantly after exposure to 10 mM Zn for 12 h, Cd for 24 h, Cu for 48 h. and was exposure to 37 degrees C for 24 11 sea water (C) 2009 Elsevier Ltd. All rights reserved
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据