期刊
FISH & SHELLFISH IMMUNOLOGY
卷 27, 期 4, 页码 577-584出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2009.07.010
关键词
Neuronal NO synthase (nNOS); Expression pattern
资金
- Foundation of Science and Technology Thriving Ocean and Fishery [A200501 G01]
- National Key Technology RD Program [200713AD291304]
- Foundation of Science and Technology of Guangdong Province [200713020708013]
The full length cDNA sequence for neuronal nitric oxide synthase (nNOS) gene from red drum (Sciaenops ocellatus) has been cloned, subjected to bioinformatic analysis, and examined for expression in different tissues. Red drum nNOS showed high identity to nNOS of mammals and other fish species. Notably, a unique 7-aa insertion was found in the important catalytic sites of the NO synthase domain, possibly affecting the function of red drum nNOS. Furthermore, this nNOS was expressed not only in brain but also in most of the internal organs including liver, intestine, spleen, head kidney and thymus. (C) 2009 Elsevier Ltd. All rights reserved.
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