期刊
FERTILITY AND STERILITY
卷 100, 期 1, 页码 108-+出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2013.02.053
关键词
Cytokines; decidua; miscarriage; prolactin; villi
Objective: To investigate the expression of prolactin (PRL), PRL-receptor (PRL-R), and the T(H)1 cytokines interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma) at the maternofetal interface. Design: Case-control study. Setting: University hospital unit of gynecology and obstetrics and research laboratories. Patient(s): Women undergoing suction curettage for spontaneous miscarriage (study group) and voluntary termination of pregnancy (control group) in the first trimester. Intervention(s): Samples of decidua and villi collected and histologically examined at the time of suction curettage. Main Outcome Measure(s): Evaluation of all villous samples for karyotype with only euploid cases included; detection of transcripts of PRL, PRL-R, TNF-alpha, IFN-gamma, and IL-2 by qualitative reverse-transcriptase-polymerase chain reaction (RT-PCR); investigation of pattern and site of expression by immunohistochemistry. Result(s): In both groups, PRL-R and IFN-gamma were broadly expressed. The expression of PRL was impaired or absent in the villi of the study group compared with controls. Expression of TNF-alpha was reduced, although not statistically significantly, in both decidual and villous samples of the study group. Immunohistochemical analysis showed the lack of IL-2 expression in decidual specimens of the control group versus the full expression shown in the study group. Conclusion(s): Our results highlight the correspondence between PRL expression and vital pregnancy and the involvement of the T(H)1 cytokines with different specific roles at the implantation site. Prolactin and IL-2 may reciprocally influence expression. (C) 2013 by American Society for Reproductive Medicine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据