期刊
FERTILITY AND STERILITY
卷 94, 期 6, 页码 2030-2036出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2010.02.022
关键词
Cytokines; endometrium; immune cells; inflammation; local injury
资金
- Dwek Fund for Biomedical Research
- Israel Ministry of Health
Objective: To study whether an injury-induced inflammation might be the mechanism underlying the favorable effect of endometrial biopsy on the implantation rate in in vitro fertilization (IVF) patients. Design: Controlled clinical study. Setting: A medical center IVF unit and a research institute. Patient(s): Women undergoing IVF who had previous failed treatment cycles. Intervention(s): Endometrial samples were collected from two groups of patients on day 21 of their spontaneous menstrual cycle. The experimental, but not the control group underwent prior biopsy treatment on days 8 or/and 11 to 13 of that same cycle. Main Outcome Measure(s): Abundance of immune cells, cytokines/chemokines level, correlation between these parameters and pregnancy outcome. Result(s): A statistically significantly higher amount of macrophages/dendritic cells (HLA-DR+ CD11c(+) cells) and elevated proinflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha), growth-regulated oncogene-alpha (GRO-alpha), interleukin-15 (IL-15), and macrophage inflammatory protein 1B (MIP-1B), were detected in day-21 endometrial samples of the experimental group. A direct stimulatory effect of TNF-alpha on MIP-1B, GRO-alpha, and IL-15 messenger RNA (mRNA) expression was demonstrated. A positive correlation was found between the levels of macrophages/dendritic cells, MIP-1B expression, and TNF-alpha expression and the pregnancy outcome. Conclusion(s): A biopsy-induced inflammatory response may facilitate the preparation of the endometrium for implantation. Increased MIP-1B expression could possibly serve for prediction of implantation competence. (Fertil Steril (R) 2010;94:2030-6. (C) 2010 by American Society for Reproductive Medicine.)
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