期刊
FERTILITY AND STERILITY
卷 93, 期 3, 页码 986-994出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2008.10.030
关键词
Coculture; differentiation; germ cell; human embryonic stem cell; Vasa
资金
- National University of Singapore [R-174-000-089-133]
- National Medical Research Council, Singapore [R-174-000-103-213]
Objective: To explore several culture systems that may prove efficient in driving human embryonic stein cells (hESCs) toward a germ cell lineage. Design: Embryoid bodies (EBs) derived from a female hESC line [HES-3 (XX)] and male hESC line [HES-4 (XY)] were cultured in six different culture conditions: [1] mitotically inactivated porcine ovarian fibroblasts (POF), [2] 100% conditioned medium from POF, [3] 50% conditioned medium from POF, [4] forskolin, [5] trans-retinoic acid (RA), and [6] forskolin and RA. Setting: Department of Obstetrics and Gynecology, National University of Singapore Research Laboratories, Singapore. Patient(s): None. Intervention(S): None. Main Outcome Measure(S): None. Result(s): Expression data for both HES-3 and HES-4 differentiating cultures strongly indicated that inactivated POFs encouraged differentiation of hESC EBs into a germ cell lineage. VASA and other germ cell markers were found to be elevated in all six culture conditions. Conclusion(s): Overall, POFs proved to be the best system for initiating germ cell differentiation, as shown by increases in the expression of several germ cell marker genes in EBs that Were cocultured with POFs. (Fertil Steril (R) 2010:93:986-94. (C)2010 by American Society for Reproductive Medicine.)
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