期刊
FERTILITY AND STERILITY
卷 93, 期 7, 页码 2337-2347出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2009.01.111
关键词
Primordial follicles; VEGF-A receptors; immunohistochemistry; in situ hybridization; RT-PCR
Objective: To investigate the expression of vascular endothelial growth factor A (VEGF-A) and that its two receptors (VEGFR1, VEGFR2) in human preantral follicles. Design: Immunohistochemical, in situ hybridization, and reverse transcriptase polymerase chain reaction (RT-PCR) study of the expression of the VEGF-A system in human ovaries. Setting: Major tertiary-care academic center. Patient(s): Twenty-two patients who underwent pregnancy terminations at 21-35 gestational weeks and 29 girls/women aged 5-9 years who underwent ovarian laparoscopies. Intervention(s): None. Main Outcome Measure(s): Laboratory analysis of human ovarian specimens. Result(s): Immunhistochemistry and in situ hybridization revealed the expression of the proteins and mRNA transcripts for VEGFR1 and VEGFR2 in oocytes, granulosa cells, and stroma cells from fetuses and girls/women. The protein for VEGF-A was detected immunohistochemically in oocytes, granulosa cells, and stroma cells from fetuses and girls. VEGF-A and VEGFR1 proteins were expressed more strongly than VEGFR2. VEGF-A(121), VEGF-A(165), and VEGF-A(189) isoforms were identified by RT-PCR in the ovarian samples from fetuses and women. Conclusion(s): The presence of the VEGF-A receptors, particularly in the granulosa cells, suggests that VEGF-A might be involved in proliferation initiation of primordial follicles or play an as yet unknown role in human preantral follicles. (Fertil Steril (R) 2010; 93: 2337-47. (C)2010 by American Society for Reproductive Medicine.)
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