期刊
FEMS YEAST RESEARCH
卷 12, 期 7, 页码 741-747出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1567-1364.2012.00823.x
关键词
protein turnover; C13 labelling; ATP costs
资金
- European Research Council [247013]
- Knut and Alice Wallemberg Foundation
- Chalmers Foundation
- European Research Council (ERC) [247013] Funding Source: European Research Council (ERC)
We developed a method for the quantification of protein turnover rates using 13C-labeled substrates combined with the analysis of the labeling patterns of proteinogenic amino acids. Using this method, the specific amino acid turnover rates between proteins and the pool of free amino acids were determined for eight different amino acids (alanine, valine, proline, aspartic acid, glycine, leucine, isoleucine, and threonine) in two Saccharomyces cerevisiae strains (CEN.PK 113-7D and YSBN2). Furthermore, proteasome activities were compared for both strains. Both results confirmed the hypothesis of a higher protein turnover rates in CEN.PK 113-7D, which was generated in a previous comparative systems biology study of these two yeast strains. The ATP costs associated with the observed differences in protein turnover were quantified and could explain accurately the differences in biomass yield between both strains that are observed in chemostat cultures. The percentage of maintenance ATP associated with protein polymerization (polymerization for growth and re-polymerization because of turnover) and degradation was estimated to be 72% for YSBN2 and 79% for CEN.PK 113-7D, which makes these processes the dominant nonbiosynthetic drain of ATP in living cells, and hence, it represents an energetic parameter of great relevance.
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