期刊
FEMS YEAST RESEARCH
卷 12, 期 2, 页码 171-182出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1567-1364.2011.00775.x
关键词
arabinose fermentation; evolutionary engineering; Saccharomyces cerevisiae; stress resistance; xylose fermentation
资金
- Turkish State Planning Organization (DPT)
- Turkish Scientific and Technological Research Council (TUBITAK) [105T314, 107T284, 109T638]
- Istanbul Technical University (ITU) [30108]
- ITU-Institute of Science and Technology [33237, 34200, 33567]
- FEMS [2009-2]
This article reviews evolutionary engineering of Saccharomyces cerevisiae. Following a brief introduction to the rational metabolic engineering approach and its limitations such as extensive genetic and metabolic information requirement on the organism of interest, complexity of cellular physiological responses, and difficulties of cloning in industrial strains, evolutionary engineering is discussed as an alternative, inverse metabolic engineering strategy. Major evolutionary engineering applications with S. cerevisiae are then discussed in two general categories: (1) evolutionary engineering of substrate utilization and product formation and (2) evolutionary engineering of stress resistance. Recent developments in functional genomics methods allow rapid identification of the molecular basis of the desired phenotypes obtained by evolutionary engineering. To conclude, when used alone or in combination with rational metabolic engineering and/or computational methods to study and analyze processes of adaptive evolution, evolutionary engineering is a powerful strategy for improvement in industrially important, complex properties of S. cerevisiae.
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