期刊
FEMS YEAST RESEARCH
卷 8, 期 3, 页码 451-459出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1567-1364.2008.00358.x
关键词
Malassezia pachydermatis; chitin synthase-2 gene (chs-2); first internal transcribed spacer (ITS-1); phospholipase; single-strand conformation polymorphism (SSCP) analysis
Malassezia pachydermatis isolates (n=185) from skin sites from dogs (n=30) were characterized genetically and biochemically following in vitro culture. Two regions in the chitin synthase-2 gene (chs-2) and the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA were sequenced, and the phospholipase activity of each isolate was assessed. Three chs-2 (i.e. A(c), B-c and C-c) and eight ITS-1 (i.e. A(I1), A(I2), A(I3), A(I4), B-I1, C-I1, C-I2 and C-I3) sequence types were defined for all 185 samples. The findings revealed that multiple M. pachydermatis genotypes/subgenotypes could be cultured from healthy dogs or from dogs with single or multiple, generalized skin lesions. Subgenotypes A(I1) and B-I1 were associated with all skin sites of dogs sampled, whereas subgenotype C-I2 was mostly linked to a particular location. Isolates derived from skin lesions showed a significantly higher phospholipase activity compared with those from skin sites with no detectable lesions. Genotype B was mainly cultured from healthy skin; only four isolates (9.3%) had low phospholipase activity, whereas other genotypes/subgenotypes were predominantly associated with skin lesions and had a high phospholipase activity. The results of the present study suggest that the distribution pattern of particular genotypes or subgenotypes of M. pachydermatis on the skin of dogs relates to the affinity of the yeast to the host and to particular skin sites.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据