期刊
FEMS MICROBIOLOGY LETTERS
卷 353, 期 1, 页码 57-62出版社
OXFORD UNIV PRESS
DOI: 10.1111/1574-6968.12409
关键词
mechanism of antibacterial activity; lichen-forming fungi; inhibition of bacterial growth; radioactive precursors incorporation
类别
资金
- University of Gdansk [DS/L140-4-0114-12]
- Marie Curie European Reintegration Grant within 7th European Community Framework Programme [239343]
Usnic acid, a compound produced by various lichen species, has been demonstrated previously to inhibit growth of different bacteria and fungi; however, mechanism of its antimicrobial activity remained unknown. In this report, we demonstrate that usnic acid causes rapid and strong inhibition of RNA and DNA synthesis in Gram-positive bacteria, represented by Bacillus subtilis and Staphylococcus aureus, while it does not inhibit production of macromolecules (DNA, RNA, and proteins) in Escherichia coli, which is resistant to even high doses of this compound. However, we also observed slight inhibition of RNA synthesis in a Gram-negative bacterium, Vibrio harveyi. Inhibition of protein synthesis in B.subtilis and S.aureus was delayed, which suggest indirect action (possibly through impairment of transcription) of usnic acid on translation. Interestingly, DNA synthesis was halted rapidly in B.subtilis and S.aureus, suggesting interference of usnic acid with elongation of DNA replication. We propose that inhibition of RNA synthesis may be a general mechanism of antibacterial action of usnic acid, with additional direct mechanisms, such as impairment of DNA replication in B.subtilis and S.aureus.
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