期刊
FEMS MICROBIOLOGY LETTERS
卷 345, 期 2, 页码 132-140出版社
OXFORD UNIV PRESS
DOI: 10.1111/1574-6968.12196
关键词
Rv2506; MSMEG_4718; accA1; accD1; transcriptional regulation; microarray
类别
资金
- Medical Research Council [U117581288] Funding Source: Medline
- Wellcome Trust [073237] Funding Source: Medline
This study describes how bkaR, a highly conserved mycobacterial TetR-like transcriptional repressor, regulates a number of nearby genes that have associations with branched-chain keto-acid metabolism. bkaR (MSMEG_4718) was deleted from the nonpathogenic species Mycobacterium smegmatis, and changes in global gene expression were assessed using microarray analysis and reporter gene studies. bkaR was found to directly control the expression of 10 genes in M.smegmatis, and its ortholog in Mycobacterium tuberculosis (Rv2506) is predicted to control at least 12 genes. A conserved operator motif was identified, and binding of purified recombinant M.tuberculosis BkaR to the motif was demonstrated. Analysis of the stoichiometry of binding showed that BkaR binds to the motif as a dimer.
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