期刊
FEMS MICROBIOLOGY LETTERS
卷 330, 期 1, 页码 56-65出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2012.02533.x
关键词
Staphylococcus epidermidis; planktonic; biofilm; adhesion; intracellular survival; cytokines; mononuclear cells
类别
资金
- MRC [G0801929] Funding Source: UKRI
- Medical Research Council [G0801929] Funding Source: researchfish
- Medical Research Council [G0801929] Funding Source: Medline
Staphylococcus epidermidis is a leading cause of hospital-acquired and biofilm-associated infections. Interactions of peripheral blood mononuclear cells (PBMCs) and monocyte-derived macrophages with planktonic or biofilm phase S.epidermidis cells were studied. Biofilm phase bacteria exhibited higher attachment, as well as, a 10-fold higher intracellular survival in monocyte-derived macrophages than their planktonic counterparts. Stimulation of PBMCs and monocyte-derived macrophages was performed with live or formalin-fixed bacterial cells. Supernatant concentration of selected cytokines was measured by Luminex (R) xMAP(TM) technology at different time points. As compared to planktonic phase, biofilm phase bacteria elicited lower amounts of proinflammatory cytokines and Th1 response cytokines, such as TNFa, IL-12p40, IL-12p70 and IFN-?, whereas they enhanced production of IL-8, GM-CSF and IL-13. This phenomenon was independent of formalin pretreatment. Taken together, these results may contribute to interpretation of observed silent course of biofilm-associated infections.
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