期刊
FEMS MICROBIOLOGY LETTERS
卷 327, 期 1, 页码 9-14出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2011.02450.x
关键词
GASP mutants; Shewanella oneidensis MR-1; glucose use; mutator bacteria; microbial fuel cells
类别
资金
- Office of Naval Research through NRL [62123N]
- NRL [61153N]
Shewanella oneidensis MR-1 has conventionally been considered unable to use glucose as a carbon substrate for growth. The genome sequence of S. oneidensis MR-1 however suggests the ability to use glucose. Here, we demonstrate that during initial glucose exposure, S. oneidensis MR-1 quickly and frequently gains the ability to utilize glucose as a sole carbon source, in contrast to wild-type S. oneidensis, which cannot immediately use glucose as a sole carbon substrate. High-performance liquid chromatography and 14C glucose tracer studies confirm the disappearance in cultures and assimilation and respiration, respectively, of glucose. The relatively short time frame with which S. oneidensis MR-1 gained the ability to use glucose raises interesting ecological implications.
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